Pgem t easy ampicillin

experiment, the TcPR-4b cDNA (from cacao-M. perniciosa interaction library) was cloned into the pGem-T Easy vector then subcloned on the pCambia binary vector 1390.T E C H N IC A L N O T E Development of single sequence repeat markers for the ant. puriÞed and ligated into a clon ing vector using pGEM -T Easy Vector II.

Specific cpb copies within the Leishmania donovani complex

Macrobrachium rosenbergii nodavirus infection in M. rosenbergii (de Man) with white tail disease cultured. amplicons were individually cloned into pGEM-T.

Insertion polymorphism of transposable elements and

Ligation of purified PCR products were performed in triplicate, as described in the pGEM®-T Easy Vector System Technical Manual #TM042. and 125µg/ml ampicillin.

Microsatellite DNA markers for the pea aphid Acyrthosiphon

Transgene silencing in grapevines transformed with GFLV

Molecular biology protocols (in C. Dauphin-Villemant lab. Preparation of Petri dishes with LB+ampicillin. cloning of PCR products in pGEM-T Easy.

Macrobrachium rosenbergii nodavirus infection in M

Hepatitis B Subviral Envelope Particle Morphogenesis and

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PCR products of different size were cloned using pGEM T-Easy vector. After transformation into E. coli DH5a com-.

Pyrethroid and DDT cross-resistance inis correlated with

Reverse primer: PCI PCR with PP3-PCI primer pair PCR-product ligated to pGEM-T Easy vector Transform to E. coli DH 5-α strain Cleaning the plasmid and.The PCR product was cloned into pGEM-T Easy (Promega) and sequenced in both directions using primers t7 and SP6, which flank the cloning region in the pGEM-T Easy.UNIVERSITE DE STRASBOURG THESE DE DOCTORAT Présentée à la FACULTE DES SCIENCES DE LA VIE. I.9.8.4 Cloning by pGEM®-T Easy Vector System I 84 I.9.8.

On the identity and origin of the Mediterranean invasive Caulerpa racemosa (Caulerpales, Chlorophyta). PCR products were then cloned into pGem-T Easy.

Automated plasmid DNA purification in 96-well plate and 8

pGEM®-T Easy Vector System II 20 reactions A1380 Includes: • 1.2µg pGEM.

Three independent mutations in the TSC2 gene in a family

Semipermeable species boundaries between Anopheles

This PDF file is made available from the Glomeromycota

into pGem T-easy vector (Promega,) and sequenced. PCR products were sequenced in both directions with slightly nested primers AegF1 50-AACTTACTCATTTCCAT-.The PCR product was inserted into pGEM®-T (pGEM®-T Easy Vector System, Promega) and then into the Bam HI restriction site of the pSFV vector (Invitrogen).pGEM-T Easy vector (Promega), and the plasmid trans-formed into Escherichia coli [email protected] supercompetent cells (XL1 blue, Correspondence: Marina C. Caillaud.Hepatitis B Subviral Envelope Particle Morphogenesis and Intracellular Tra cking Romuald Patient, Christophe Hourioux, Pierre-Yves Sizaret, Sylvie Trassard.®fragments were cloned into the pGEM -T Easy vector and subsequently the complete sequence of BtMV-G was determined. In addition, four cDNA clones generated by RT-.

the Isis sample. The amplified products were cloned using the pGEM-T Vector System (Promega). To elirmnate.pGEM-T Easy vector (Promega, Charbonnieres, France). Escherichia coli cells were transformed with the resulting. 200 µl of LB supplemented with ampicillin (100 µg ml.The amplified fragment was cloned into pGEM-T Easy (Promega) forming pGEM-ITR. Genome-Wide Identification of Ampicillin Resistance Determinants in Enterococcus.

On the identity and origin of the Mediterranean invasive

Easy Promoter Prediction Program is a tool for the identification of the core region of a eukaryotic gene promoter. It uses universal properties of the promoter.Purification of blunt-ended plasmid and addition of T-overhang 1) 1- In a 1.5 mL microcentrifuge tube, digest 10µg of plasmid with either.

Molecular characterization of a novel Sunflower chlorotic