experiment, the TcPR-4b cDNA (from cacao-M. perniciosa interaction library) was cloned into the pGem-T Easy vector then subcloned on the pCambia binary vector 1390.T E C H N IC A L N O T E Development of single sequence repeat markers for the ant. puriÞed and ligated into a clon ing vector using pGEM -T Easy Vector II.
Specific cpb copies within the Leishmania donovani complexMacrobrachium rosenbergii nodavirus infection in M. rosenbergii (de Man) with white tail disease cultured. amplicons were individually cloned into pGEM-T.
Insertion polymorphism of transposable elements andLigation of purified PCR products were performed in triplicate, as described in the pGEM®-T Easy Vector System Technical Manual #TM042. and 125µg/ml ampicillin.
Microsatellite DNA markers for the pea aphid Acyrthosiphon
Transgene silencing in grapevines transformed with GFLVMolecular biology protocols (in C. Dauphin-Villemant lab. Preparation of Petri dishes with LB+ampicillin. cloning of PCR products in pGEM-T Easy.
Macrobrachium rosenbergii nodavirus infection in M
Hepatitis B Subviral Envelope Particle Morphogenesis and
PowerPoint bemutató - bordeaux.inra.frPCR products of different size were cloned using pGEM T-Easy vector. After transformation into E. coli DH5a com-.
Pyrethroid and DDT cross-resistance inis correlated withReverse primer: PCI PCR with PP3-PCI primer pair PCR-product ligated to pGEM-T Easy vector Transform to E. coli DH 5-α strain Cleaning the plasmid and.The PCR product was cloned into pGEM-T Easy (Promega) and sequenced in both directions using primers t7 and SP6, which flank the cloning region in the pGEM-T Easy.UNIVERSITE DE STRASBOURG THESE DE DOCTORAT Présentée à la FACULTE DES SCIENCES DE LA VIE. I.9.8.4 Cloning by pGEM®-T Easy Vector System I 84 I.9.8.
On the identity and origin of the Mediterranean invasive Caulerpa racemosa (Caulerpales, Chlorophyta). PCR products were then cloned into pGem-T Easy.
Automated plasmid DNA purification in 96-well plate and 8pGEM®-T Easy Vector System II 20 reactions A1380 Includes: • 1.2µg pGEM.
Three independent mutations in the TSC2 gene in a family
Semipermeable species boundaries between Anopheles
This PDF file is made available from the Glomeromycotainto pGem T-easy vector (Promega,) and sequenced. PCR products were sequenced in both directions with slightly nested primers AegF1 50-AACTTACTCATTTCCAT-.The PCR product was inserted into pGEM®-T (pGEM®-T Easy Vector System, Promega) and then into the Bam HI restriction site of the pSFV vector (Invitrogen).pGEM-T Easy vector (Promega), and the plasmid trans-formed into Escherichia coli [email protected] supercompetent cells (XL1 blue, Correspondence: Marina C. Caillaud.Hepatitis B Subviral Envelope Particle Morphogenesis and Intracellular Tra cking Romuald Patient, Christophe Hourioux, Pierre-Yves Sizaret, Sylvie Trassard.®fragments were cloned into the pGEM -T Easy vector and subsequently the complete sequence of BtMV-G was determined. In addition, four cDNA clones generated by RT-.
the Isis sample. The amplified products were cloned using the pGEM-T Vector System (Promega). To elirmnate.pGEM-T Easy vector (Promega, Charbonnieres, France). Escherichia coli cells were transformed with the resulting. 200 µl of LB supplemented with ampicillin (100 µg ml.The amplified fragment was cloned into pGEM-T Easy (Promega) forming pGEM-ITR. Genome-Wide Identification of Ampicillin Resistance Determinants in Enterococcus.